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KMID : 0617320000090010108
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Journal of Pharmacetical Sceiences Ewha Womans University
2000 Volume.9 No. 1 p.108 ~ p.111
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Identification of the Self-Interaction of Rat TCTP/lgE-Dependent Histamine-Releasing Factor Using Yeast Two-Hybrid System
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Yoon, Taesook
Jung, Jaehoon/Kim, Minjeong/Lee, Kang Man/Choi, Eung Chil/Lee, Kyunglim
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Abstract
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To further understand the biological function of translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor (HRF), the yeast two-hybrid system was used to screen interacting molecules. We isolated cDNA clones coding for TCTP/HRF, suggesting that it may have a self-interacting property. Domain mapping of the interaction revealed that the C-terminal region of residue 126-172 is involved in self-interaction. The self-interacting property of TCTP/HRF was further supported by FPLC gel-filtration chromatography and coimmunoprecipitation analysis from transfected COS-7 cells. Our data suggests that TCTP/HRF may have a potential to self-interact through the C-terminal region, and the self-interaction property may be related to its biological function.
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